Mitochondrial calcium signaling is essential for power metabolism and calcium homeostasis, however monitoring it precisely is difficult on account of pH fluctuations. Current GECIs are sometimes delicate to pH adjustments, resulting in artifacts and inaccurate measurements. Moreover, the restricted availability of cyan fluorescent GECIs hinders multiplexed imaging.
Constructing on the intense cyan fluorescent protein mTurquoise2, TurCaMP provides an inverse response to Ca2+ transients and is insensitive to pH adjustments throughout the physiological vary. This makes it a worthwhile software for finding out mitochondrial calcium signaling with excessive signal-to-noise ratio and minimal background noise.
TurCaMP’s pH insensitivity and cyan fluorescence allow multiplexed imaging of calcium indicators in several mobile compartments, opening new avenues for finding out complicated calcium-dependent physiological occasions.
This analysis showcases the potential of computational modeling and experimental validation in growing novel GECIs. TurCaMP represents a big development in calcium imaging instruments and holds nice promise for advancing our understanding of mitochondrial calcium signaling and its function in numerous mobile processes and illnesses.
Supply:
Journal reference:
Gu, W., et al. (2024). A vibrant cyan fluorescence calcium indicator for mitochondrial calcium with minimal interference from physiological pH fluctuations. Biophysics Studies. doi.org/10.52601/bpr.2024.240001.